Biotinylated Anti-PEG monoclonal IgM Antibody (ANPEG-1)
ANPEG-1 mouse monoclonal IgM against PEG has been successfully demonstrated as a useful tool not only in immunogenicity assays, but also in pharmacokinetic (PK) assays for the detection of PEG or intact PEGylated drugs.
ANPEG-1 detector can be biotinylated and a streptavidin-HRP conjugate can be used to label in situ the ANPEG-1 detector in sandwich complexes formed in a PK sandwich ELISA as shown in Figure 1.
Figure 1. Biotinlyated ANPEG-1 used as the detector antibody in a PK ELISA for a PEGylated Protein Drug. Streptavidin conjugate to HRP reacts with biotinylated ANPEG-1 for subsequent signal generation.
It can also be used as the capture or detector antibody in a PK sandwich ELISA. In Figure 2, ANPEG-1 is used as a capture antibody directly coated onto a regular 96 well plate (Precoated ANPEG-1 plates, P/N 11-5001). In this PK ELISA, the resulting sandwich complex will consist of ANPEG-1 capture antibody attaching the PEGylated protein drug via its PEG chain, and an enzyme-labeled detector antibody against the protein moiety of the PEGylated drug.
Figure 2. ANPEG-1 used as the capture antibody in an PK assay for a PEGylated Drug. The IgM is directly coated on a 96-well plate. The detector antibody recognized the drug portion of the PEGylated drug.