Product Brochure
Anti-PEG Monoclonal IgM Antibody (ANPEG-1)
Your ideal choice for a detector and capture antibody.
ANPEG-1 mouse monoclonal IgM against PEG has been successfully demonstrated as a useful tool not only in immunogenicity assays, but also in pharmacokinetic (PK) assays for the detection of PEG or intact PEGylated drugs.
ANPEG-1 detector can be biotinylated and a streptavidin-HRP conjugate can be used to label in situ the ANPEG-1 detector in sandwich complexes formed in a PK sandwich ELISA as shown in Figure 1.
Figure 1. Biotinlyated ANPEG-1 used as the detector antibody in a PK ELISA for a PEGylated Protein Drug. Streptavidin conjugate to HRP reacts with biotinylated ANPEG-1 for subsequent signal generation.
It can also be used as the capture or detector antibody in a PK sandwich ELISA. In Figure 2, ANPEG-1 is used as a capture antibody directly coated onto a regular 96 well plate (Precoated ANPEG-1 plates, P/N 11-5001). In this PK ELISA, the resulting sandwich complex will consist of ANPEG-1 capture antibody attaching the PEGylated protein drug via its PEG chain, and an enzyme-labeled detector antibody against the protein moiety of the PEGylated drug.
Figure 2. ANPEG-1 used as the capture antibody in an PK assay for a PEGylated Drug. The IgM is directly coated on a 96-well plate. The detector antibody recognized the drug portion of the PEGylated drug.
Figure 3 is an example of ANPEG-1 used as the detector antibody in a PK sandwich ELISA for PEGylated protein drugs. It can be conjugated directly to an enzyme such as horseradish peroxidase (HRP) and be combined with a capture antibody specific to the protein moiety of a PEGylated protein drug.
Figure 3. ANPEG-1 used as the detector antibody directly labeled with HRP in a PK assay for a PEGylated Drug.
Ordering Information:
Anti-PEG Monoclonal IgM Antibody (ANPEG-1)
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Description: Mouse monoclonal anti-PEG antibody/PEC antibody specifically binds to the repeating unit of PEG polymer.
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Type: Primary
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Host: Mouse
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Antigen: PEG
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Conjugation: Unconjugated
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Clonality: Monoclonal
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Shipped in buffer
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Storage Buffer: 10mM PBS
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Storage Temperature: Store at -80°C prior to opening.
Anti-PEG monoclonal IgM Antibody (ANPEG-1) (100 µg)
Product No. 90-1010-100UG
$350.00
Anti-PEG monoclonal IgM Antibody (ANPEG-1) (500 µg)
Product No. 90-1010-500UG
$799.00
Anti-PEG monoclonal IgM Antibody (ANPEG-1) (1 mg)
Product No. 90-1010-1MG
$1490.00
Anti-PEG Monoclonal IgM Ab without stabilizer (ANPEG-1)
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Description: Mouse monoclonal anti-PEG antibody/PEC antibody specifically binds to the repeating unit of PEG polymer.
-
Type: Primary
-
Host: Mouse
-
Antigen: PEG
-
Conjugation: Unconjugated
-
Clonality: Monoclonal
-
Shipped in buffer
-
Storage Buffer: 10mM PBS
-
Storage Temperature: Store at -80°C prior to opening.
Anti-PEG Monoclonal IgM Ab without stabilizer (ANPEG-1) (100 µg)
Product No. 90-1061-100UG
$350.00
Anti-PEG Monoclonal IgM Ab without stabilizer (ANPEG-1) (500 µg)
Product No. 90-1061-500UG
$799.00
Anti-PEG Monoclonal IgM Ab without stabilizer (ANPEG-1) (1 mg)
Product No. 90-1061-1MG
$1490.00